How to elute dna in 1 step using syringe glass-membrane filter method? - syringe filter
try to find a better composition of the eluted elution buffer to the DNA in a single step ... without the inconvenience of multiple ELUTION .....
Friday, January 15, 2010
Syringe Filter How To Elute Dna In 1 Step Using Syringe Glass-membrane Filter Method?
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A ghetto of DNA with the production of phenol and chloroform in a 1:1 mixture of phenol: chloroform: isoamyl alcohol with their DNA. Mix thoroughly. Remove the aqueous phase into the other tube, an equal volume of ice-cold 100% isopropanol and mix.
ReplyDeleteWhen the DNA with a high proportion, it may take a Pasteur pipette, the end of melting in the flame, making the hair a little thin hooks and then put it in your solution and spin ... Tail-DNA on the glass.
After breaking a small hook in a tube with TE buffer in a few, and incubated at 42C for half an hour or so. If you desperately want to solve the glass (es) will not hurt to be filtered through a high-quality, sterile bag. Mesh.
This protocol will only work if you have a ton of DNA without the addition of a salt such as sodium acetate, lithium acetate, etc. .. that the DNA is not much precipitation.
You can search the site for the preparations Qiagen genomic DNA ... take less than 15 minutes and resulted in consistently good quality / high-throughput DNA.